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anti centrin 2  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti centrin 2
    Anti Centrin 2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+centrin+2/Centrin-2+Antibody/pmc12272147-32-44-48
    Average 93 stars, based on 17 article reviews
    anti centrin 2 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Membrane:

    Article Title: Centrosome‑, mitotic spindle‑ and cytokinetic bridge‑specific compartmentalization of AGO2 protein in human liver cells undergoing mitosis: Non‑canonical, RNAi‑dependent, control of local homeostasis.
    Article Snippet: Immunofluorescence. lX‐2 and HepG2 cells (6x104; ~80% confluency) were cultured on 10 mm round coverslips and fixed in 4% paraformaldehyde (cat. no. P6148; Merck KGaA) solution for 10 min at room temperature. .. The cells were membrane‐permeabilized by incubation in 0.1% Triton‐X 100 (cat. no. 1086031000; Merck KGaa), followed by blocking with 5% BSa (cat. no. a7906; Merck KGaa) for 1 h at room temperature. cells were exposed to anti‐aGo2 (cat. no. ab57113; abcam) (1:100), anti‐dicer (double‐stranded rna endoribonuclease) (cat. no. nBP1‐06520; novus Biologicals, llc) (1:100), anti‐TrBP (transactivation response element rna‐binding protein; cat. no. ab180947; abcam) (1:100), anti‐uPF1 (up‐frameshift protein 1) (cat. no. 12040, cell Signalling Technology, inc.; 1:100), anti‐centrin‐2 (cat. no. 2091, cell Signalling Technology, inc.) (1:200), anti‐peri‐centriolar‐material (PcM)‐1 (cat. no. 5213, cell Signalling Technology, inc) (1:200), anti‐γ‐tubulin (cat. no. T5192; Merck KGaa) (1:200) and anti‐α‐tubulin (cat. no. 3873, cell Signalling Technology, inc.) (1:100) primary antibodies, overnight at 4 ̊C. .. Cells were incubated with secondary antibodies [goat anti‐mouse (cat. no. a‐11004; Invitrogen; Thermo Fisher Scientific, Inc.) and anti‐rabbit IgG (H+l)‐alexa FluorTM 568 (cat. no. a‐11011; invitrogen, Thermo Fisher Scientific, Inc.) and anti‐mouse IgG (H+L)‐Alexa Fluor 488 (cat. no. A‐28175; Invitrogen; Thermo Fisher Scientific, inc.) and donkey anti‐rabbit igG (H+l)‐alexa Fluor 488 (cat. no. A‐21206, Invitrogen; Thermo Fisher Scientific, Inc.)] (1:500) for 1 h at room temperature.

    Incubation:

    Article Title: Centrosome‑, mitotic spindle‑ and cytokinetic bridge‑specific compartmentalization of AGO2 protein in human liver cells undergoing mitosis: Non‑canonical, RNAi‑dependent, control of local homeostasis.
    Article Snippet: Immunofluorescence. lX‐2 and HepG2 cells (6x104; ~80% confluency) were cultured on 10 mm round coverslips and fixed in 4% paraformaldehyde (cat. no. P6148; Merck KGaA) solution for 10 min at room temperature. .. The cells were membrane‐permeabilized by incubation in 0.1% Triton‐X 100 (cat. no. 1086031000; Merck KGaa), followed by blocking with 5% BSa (cat. no. a7906; Merck KGaa) for 1 h at room temperature. cells were exposed to anti‐aGo2 (cat. no. ab57113; abcam) (1:100), anti‐dicer (double‐stranded rna endoribonuclease) (cat. no. nBP1‐06520; novus Biologicals, llc) (1:100), anti‐TrBP (transactivation response element rna‐binding protein; cat. no. ab180947; abcam) (1:100), anti‐uPF1 (up‐frameshift protein 1) (cat. no. 12040, cell Signalling Technology, inc.; 1:100), anti‐centrin‐2 (cat. no. 2091, cell Signalling Technology, inc.) (1:200), anti‐peri‐centriolar‐material (PcM)‐1 (cat. no. 5213, cell Signalling Technology, inc) (1:200), anti‐γ‐tubulin (cat. no. T5192; Merck KGaa) (1:200) and anti‐α‐tubulin (cat. no. 3873, cell Signalling Technology, inc.) (1:100) primary antibodies, overnight at 4 ̊C. .. Cells were incubated with secondary antibodies [goat anti‐mouse (cat. no. a‐11004; Invitrogen; Thermo Fisher Scientific, Inc.) and anti‐rabbit IgG (H+l)‐alexa FluorTM 568 (cat. no. a‐11011; invitrogen, Thermo Fisher Scientific, Inc.) and anti‐mouse IgG (H+L)‐Alexa Fluor 488 (cat. no. A‐28175; Invitrogen; Thermo Fisher Scientific, inc.) and donkey anti‐rabbit igG (H+l)‐alexa Fluor 488 (cat. no. A‐21206, Invitrogen; Thermo Fisher Scientific, Inc.)] (1:500) for 1 h at room temperature.

    Blocking Assay:

    Article Title: Centrosome‑, mitotic spindle‑ and cytokinetic bridge‑specific compartmentalization of AGO2 protein in human liver cells undergoing mitosis: Non‑canonical, RNAi‑dependent, control of local homeostasis.
    Article Snippet: Immunofluorescence. lX‐2 and HepG2 cells (6x104; ~80% confluency) were cultured on 10 mm round coverslips and fixed in 4% paraformaldehyde (cat. no. P6148; Merck KGaA) solution for 10 min at room temperature. .. The cells were membrane‐permeabilized by incubation in 0.1% Triton‐X 100 (cat. no. 1086031000; Merck KGaa), followed by blocking with 5% BSa (cat. no. a7906; Merck KGaa) for 1 h at room temperature. cells were exposed to anti‐aGo2 (cat. no. ab57113; abcam) (1:100), anti‐dicer (double‐stranded rna endoribonuclease) (cat. no. nBP1‐06520; novus Biologicals, llc) (1:100), anti‐TrBP (transactivation response element rna‐binding protein; cat. no. ab180947; abcam) (1:100), anti‐uPF1 (up‐frameshift protein 1) (cat. no. 12040, cell Signalling Technology, inc.; 1:100), anti‐centrin‐2 (cat. no. 2091, cell Signalling Technology, inc.) (1:200), anti‐peri‐centriolar‐material (PcM)‐1 (cat. no. 5213, cell Signalling Technology, inc) (1:200), anti‐γ‐tubulin (cat. no. T5192; Merck KGaa) (1:200) and anti‐α‐tubulin (cat. no. 3873, cell Signalling Technology, inc.) (1:100) primary antibodies, overnight at 4 ̊C. .. Cells were incubated with secondary antibodies [goat anti‐mouse (cat. no. a‐11004; Invitrogen; Thermo Fisher Scientific, Inc.) and anti‐rabbit IgG (H+l)‐alexa FluorTM 568 (cat. no. a‐11011; invitrogen, Thermo Fisher Scientific, Inc.) and anti‐mouse IgG (H+L)‐Alexa Fluor 488 (cat. no. A‐28175; Invitrogen; Thermo Fisher Scientific, inc.) and donkey anti‐rabbit igG (H+l)‐alexa Fluor 488 (cat. no. A‐21206, Invitrogen; Thermo Fisher Scientific, Inc.)] (1:500) for 1 h at room temperature.



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